| 摘要 |
The present invention provides a cancer/testis antigen which is applicable to diagnosis and immunotherapy for cancer, that is to say, to provide a cancer/testis antigen which is not expressed in normal tissues other than the testis, which is expressed in various cancers over a broad range and which induces immune response in a host, and to provide a caner/testis gene encoding the cancer/testis antigen, etc. By using the yeast Two-hybrid System, the present inventors screened a protein which specifically binds to GCF, a previously reported transcription factor, cloned a gene of a protein specifically binding to the GCF, confirmed that the D40 protein as a resulting gene product is a cancer/testis antigen which is expressed substantially only in the testis in normal human tissues whereas in cancers it is expressed in human primary cancers over a broad range derived from various tissues and cells, and found that the D40 shows high affinity with HLA and has a plurality of sequences comprising 9 or 10 amino acid residues and binding to HLA.
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