Described herein is a method of expression cloning useful for identifying and obtaining proteins involved in GLUT4 trafficking in mammalian cells and, thus, in insulin-stimulated glucose uptake by such cells. In particular, an enrichment strategy for expression cloning proteins involved in GLUT4 trafficking at the plasma membrane is described. Proteins identified by the method and uses therefore are also described.
申请公布号
WO02059299(A3)
申请公布日期
2004.02.12
申请号
WO2002US02392
申请日期
2002.01.28
申请人
WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH;THE GENERAL HOSPITAL CORPORATION;BOGAN, JONATHAN, S.;LODISH, HARVEY, F.