Method for the separation of triglycoalkaloids

摘要

In accordance with the present invention there is provided a method for the separation of a triglycoalkaloid in which the triglycoside portion comprises alpha-L-rhamnopyranosyl-(1->2gal)-O-beta-D-glucopyranosyl-(1->3gal)-beta-D-galactopyranose (or 6-deoxy-alpha-L-mannopyranosyl-(1->2)-O-[beta-D-glucopyranosyl-(1->3)]-beta-D-galactopyranoside) ('an rhamnose-glucose-galactose triglycoalkaloid') from a triglycoalkaloid in which the triglycoside portion is alpha-L-rhamnopyranosyl-(1->2glu)-O-alpha-L-rhamnopyranosyl-(1->4glu)-beta-D-glucopyranose ('a rhamnose-rhamnose-glucose triglycoalkaloid') in a mixture containing both, the method comprising the steps of: Combining the mixture with a portion of alcoholic solvent; Causing or allowing a substantial portion of the rhamnose-rhamnose-glucose triglycoalkaloid to dissolve, thereby generating an alcoholic solution substantially of the rhamnose-rhamnose-glucose triglycoalkaloid, and a solid substantially comprising the rhamnose-glucose-galactose triglycoalkaloid; and Separating the solid substantially comprising the rhamnose-glucose-galactose triglycoalkaloid from the solution substantially of the rhamnose-rhamnose-glucose triglycoalkaloid.