Method and system or detecting nucleic acids

摘要

A method for the detection of a target nucleic acid in a sample containing a mixture of nucleic acids. The method comprises (a) providing a solid surface; (b) attaching to the solid surface an oligonucleotide probe complementary to a segment of the target nucleic acid; (c) contacting the surface with the sample, thereby allowing the probe to bind the target nucleic acid; (d) incubating the bound target nucleic acid with the 4 nucleotide types and a replication biocatalyst thereby forming a multi-stranded nucleic acid assembly, wherein at least one of the nucleotide types is bound by a label; and (e) detecting the label on the multi-stranded nucleic acid assembly, thereby detecting the target nucleic acid. Also disclosed are a system for identifying a target nucleic acid sequence in a sample and a kit for use in the method.