发明名称 |
METHODS FOR DETECTING NUCLEIC ACID SEQUENCE VARIATIONS |
摘要 |
The invention employs an unlabeled signal primer comprising a 5' adapter sequence for detection of variations in nucleic acid target sequences. The detection system further comprises a reporter probe, the 3' end of which hybridizes to the complement of the 5' adapter sequence of the signal primer to produce a 5' overhang. Polymerase is used to fill in the overhang and synthesize the complement of the 5' overhang of the reporter probe. Synthesi s of the reporter probe complement is detected, either directly or indirectly, as an indication of the presence of the target.
|
申请公布号 |
CA2493609(A1) |
申请公布日期 |
2004.02.05 |
申请号 |
CA20032493609 |
申请日期 |
2003.07.25 |
申请人 |
BECTON, DICKINSON AND COMPANY |
发明人 |
WANG, SHA-SHA;THORNTON, KEITH;HELLYER, TOBIN J.;NADEAU, JAMES G. |
分类号 |
A01H5/02;C12Q1/68;(IPC1-7):C12Q1/68;G01N33/48 |
主分类号 |
A01H5/02 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|