| 摘要 |
<p>Disclosed are compounds of formula: (I) In which D is a fluorescent dye selected from an acridone and a quinacridone dye; F comprises a target bonding group selected from a carboxylic acid thioester group and a 1,2-aminothiol group; M is a group adapted for attaching to F; X is selected from hydrogen or the group: (II), wherein B is an affinity tag; and L<1> and L<2> each independently comprise a group containing from 1 to 40 linked atoms selected from carbon atoms which may optionally include one or more groups selected from -NR<1->, -O-, -CH=CH-, -CO-NH- and phenylenyl groups, where R<1> is selected from hydrogen and C1 - C4 alkyl. The invention also relates to methods that afford direct attachment of the acridone or quinacridone dye to either the N-terminus or C-terminus of a synthetic or recombinant peptide or protein, and their derivatives, in a site-specific manner, coupled with purification of the resultant labelled molecule. Site-specific protein on peptide labelling involves the reaction of a thioester group with a 1,2-aminothiol group as for native chemical ligation.</p> |
