METHOD FOR ANALYZING NUCLEIC ACID CHIP BY TIME-OF-FLIGHT SECONDARY ION MASS SPECTROMETRY

摘要

PROBLEM TO BE SOLVED: To provide an analytical method for easily and highly sensitively performing quantitative analysis and two-dimensional imaging by bringing a target nucleic acid into a hybridized state to a nucleic acid probe arranged on a nucleic acid chip in a matrix shape and simultaneously and clearly discriminating the nucleic acid probe from the target nucleic acid.

SOLUTION: A probe nucleic acid and the target nucleic acid are previously labeled with different labeling substances, for example, halogen atoms to form hybrids. Then the probe nucleic acid and the target nucleic acid are individually imaged, and quantitative analysis is performed on them via measurement on secondary ions caused by the different labeling substances labeling the probe nucleic acid and the target nucleic acid.

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