Maintaining scale factor in an instrument for reading a biopolymer array

摘要

A method, apparatus for executing the method, and computer program products for use in such an apparatus. The method includes scanning an interrogating light across multiple sites on an array package including an addressable array of multiple features of different moieties, which scanned sites include multiple array features. Signals from respective scanned sites emitted in response to the interrogating light are detected. In the subject methods, a scanner is employed in which the interrogating light and detector gain are modulated in a manner sufficient to maintain constant scale factor in the scanner despite reductions in laser power resulting from laser degradation.