| 摘要 |
<p>It is intended to provide a method of detecting a base mutation characterized by comprising, to provide the base site corresponding to a mutation site of a nucleic acid having the base mutation to be detected in the base sequence formed in the 3' side after the extension, forming a primer having a base sequence complementary to a part of the above-described nucleic acid and a base sequence, which is complementary to the base sequence immediately upstream the base site corresponding to the mutation site in the above-described base sequence formed in the 3'-side, added to the 5'-terminal side; forming a target by subjecting the primer to an extension reaction with the use of a polymerase or Klenow enzyme; converting the target into a single-stranded one by denaturation; and hybridizing the target with a probe having a base complementary to the mutation site of the above-described nucleic acid in the 3'-terminal region followed by ligation for the detection. Thus, a base species at a specific position in a base sequence can be determined and quantified, thereby quickly analyzing various mutations such as a single base mutation, a mutation in a repetitive sequence, a base defect mutation, a base insert mutation, a translocation mutation and so on.</p> |
