POLYPEPTIDES DERIVED FROM RNA POLYMERASES AND USE THEREOF

摘要

Mutant RNA polymerases of phagic origin in which the peptide chain is modified by substitution, deletion or addition of at least one amino acid, the modification having the effect of reducing the sensitivity of the RNA polymerases to the initial transcription sequence of the DNA sequence coding for the RNA, for a method for production of the RNA, or proteins coded by the RNA, from given nucleotide sequences, comprising a sequence of DNA coding for the RNA, the transcription of which is placed under the control of a promoter recognised by wild-type RNA polymerases and the mutant RNA polymerases as above. The method has a higher yield of RNA than the yield obtained when using the wild-type RNA polymerases in the presence of the same non-consensual ITS as that found in the sequence of DNA coding for the RNA.