GENE SHUFFING BY TEMPLATE SWITCHING

摘要

The present invention provides a new approach to creating novel polynucleotide sequences by point mutation and recombination in vitro of a set of parental sequences. The new polynucleotide sequences can be useful in themselves or they can be used for the templated synthesis of polymers, e.g. polypeptides composed of alpha-amino acids as occurring naturally in protein synthesis or polymers comprised of beta-amino acids or other building blocks as described (Templated molecules).