| 摘要 |
<p>A process and apparatus in which cryoprotectant in cryopreserved samples is removed with the use of turbulence and convective-dispersion around the cells within the primary cryopreservation container. Convective-dispersion is applied via the combined action of gravity and pulsatile transmembrane flow of buffer through the pores of containers fabricated from appropriate membrane material such as that disclosed in U.S. Pat. No. 5,026,342 and U.S. Pat. No. 5,261,870, as incorporated herein by reference above. By using mechanical forces to aid the process of cryoprotectant removal, the benefits of slow cryoprotectant removal are retained while the actual removal time is markedly decreased.</p> |
