ELECTROPORATION METHOD INCLUDING THE USE OF DEPRESSURIZATION/PRESSURIZATION

摘要

An object of the present invention is to establish a transformation method simpler and more rapid than conventional techniques. This object can be solv ed by a method comprising the steps of a) holding a cell under a pressure different from an atmospheric pressure, and b) placing the cell and a nuclei c acid under conditions capable of inducing electroporation. The present invention removes the necessity of culture which is conventionally required after gene transfer. Therefore, the present invention makes it possible to obtain a transformant of species which are impossible or considerably difficult to transform by conventional techniques. The simple transformation method of the present invention easily allows large-scale processing and lar ge- scale analysis for research and development in the art. In addition, the present invention triggers dramatic advances in research, potentially leadin g to the development of innovative recombinants.