Plasmid originated from bifidobacterium, recombinant expression vector using the plasmid and transformation method

摘要

The present invention relates to a new plasmid originated from (Bifidobacterium) a recombinant expression vector and transformation method using the same. More particularly, the present invention relates to a plasmid pMG1 having nucleotide sequence represented by SEQ.ID.NO. 1; (Bifidobacterium longum) MG1 including the plasmid pMG1; and a shuttle vector which can be replicated in both (Bifidobacterium) and (E. coli), and comprises (Mob) gene having nucleotide sequence represented by SEQ.ID.NO. 2. (Rep) gene having nucleotide sequence represented by SEQ.ID.NO. 3 and a selection marker. The shuttle vector and the promoter of the present invention can be used for expressing target gene without additional purification process. The protein expressed from the target gene in (Bifidobacterium) can be added to food, therefore, the protein can be used for preparing food additives or oral vaccine. Furthermore, the potential and the possibility of probiotics using (Bifidobacterium) can be promoted through the development of the shuttle vector.