GENE ENCODING L-ASPARAGINASE IN ERWINIA CAROTOVORA AND STRAIN ESCHERICHIA COLI VKPM = B-8174 AS PRODUCER OF ERWINIA CARATOVORA L-ASPARAGINASE

摘要

FIELD: biotechnology, genetic engineering, enzymology, biochemistry. SUBSTANCE: DNA fragment encoding the complete amino acid sequence of L-asparaginase from Erwinia caratovora (ECAR-LANS) is isolated and characterized. Plasmid DNA pBADLANS is constructed wherein enzyme-encoding DNA fragment is under control of regulated promotor pBAD. As result of transformation of E. coli cells with proposed recombinant plasmid and the following selection of transformed clones the new strain is prepared: producer ECAR-LANS (E. coli VKPM = B-8174) providing high yield of recombinant enzyme. The main designation of this enzyme is it using as the main component in preparing the known and development of new antitumor preparations. EFFECT: improved preparing method, valuable medicinal properties of enzyme. 2 cl, 7 dwg, 4 tbl, 4 ex