发明名称 |
Expression of recombinant fusion proteins in attenuated bacteria |
摘要 |
The invention provides a DNA molecule comprising a promoter sequence operably linked to a DNA sequence encoding first and second proteins linked by a hinge region wherein in that the promoter sequence can be one having activity which is induced in response to a change in the surrounding environment and the first protein can be Tetanus toxin C fragment or one or more epitopes thereof. The invention also provides intermediate molecules having a promoter operably linked to a DNA sequence encoding a first antigenic sequence and a hinge region, and at or adjacent the 3'-end thereof one or more restriction sites for the introduction of a second anti-genic sequence. In addition, the invention provides replicable expression vectors containing the DNA fusion proteins expressed therefrom, bacterial transformed with the vectors and the use of the bacteria, in vaccines.
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申请公布号 |
US6680182(B1) |
申请公布日期 |
2004.01.20 |
申请号 |
US19950379611 |
申请日期 |
1995.07.21 |
申请人 |
ACAMBIS RESEARCH LIMITED |
发明人 |
KHAN MOHAMMED ANJAM;VILLARREAL-RAMOS BERNARDO;HORMAECHE CARLOS ESTENIO;CHATFIELD STEVEN NEVILLE;DOUGAN GORDON |
分类号 |
C12N15/09;A61K39/00;A61K39/08;C07K14/025;C07K14/035;C07K14/09;C07K14/155;C07K14/33;C07K19/00;C12N;C12N1/21;C12N9/10;C12N15/31;C12N15/54;C12N15/62;C12R1/42;(IPC1-7):C12N15/09 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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