Method of unsupervised cell nuclei segmentation

摘要

A method of segmentation of cell nuclei is described that uses an active contours approach based on a Viterbi search algorithm. Also disclosed is an initialisation technique that maximises the probability of a correct segmentation. The invention also includes a method to classify cell nuclei according to the difficulty of correct segmentation. Nuclei that are difficult to segment can be rejected to minimise the probability of false positives. The probability of false positives can be driven close to 0%.