IDENTIFICATION METHOD OF STREPTOMYCES HAVING RIFAMPIN RESISTANCE OR SENSITIVITY USING RPOB GENE, AND PRIMER SPECIFIC TO STREPTOMYCES HAVING RIFAMPIN RESISTANCE OR SENSITIVITY

摘要

PURPOSE: An identification method of Streptomyces having rifampin resistance or sensitivity using rpoB gene, and a primer specific to Streptomyces having rifampin resistance or sensitivity are provided, thereby simplifying the method, decreasing the costs therefor and improving the accuracy thereof. CONSTITUTION: An identification method of Streptomyces having rifampin resistance or sensitivity is characterized by using the nucleotide sequence difference encoding an amino acid of a rpoB gene of Streptomyces sp. corresponding to the 352th amino acid in RNA polymerase subunit B of Streptomyces coelicolor, wherein the nucleotide sequence encoding the amino acid is 258-bp to 260-bp of nucleotide sequence from the 5-terminal of a 352-bp fragment obtained by amplifying the rpoB gene of Streptomyces sp. using a pair of primers having the nucleotide sequences of SEQ ID NO: 1 and SEQ ID NO: 2.