发明名称 |
METHODS OF TREATING MICROBIAL INFECTIONS IN HUMANS AND ANIMALS |
摘要 |
A method of treating a subject with a microbially-based infection, comprisin g the administration of a compound to the subject. The compound is able to decrease ATP levels in the microbe by at least 10% compared to controls afte r 24 hours in an in vitro test, without killing mammalian cells during the sam e time period. The decrease in ATP levels is measured by: (1) removing the cel ls from the testing location and putting them on ice; (2) harvesting the cells at 4 degrees C by centrifugation and disrupting it with bead-beating in an ATP extraction buffer; (3) removing cellular debris by centrifugation at 4 degre es C, leaving an ATP-containing supernatant; (4) measuring the amount of ATP present in the supernatant by a bioluminescence assay at 4 degrees C.</SDOAB >
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申请公布号 |
CA2491573(A1) |
申请公布日期 |
2004.01.15 |
申请号 |
CA20032491573 |
申请日期 |
2003.07.09 |
申请人 |
FASGEN, LLC.;THE JOHNS HOPKINS UNIVERSITY |
发明人 |
HUGHES, MINERVA AMORETTE;TOWNSEND, CRAIG A.;DICK, JAMES D.;PARRISH, NICOLE M. |
分类号 |
C07D333/32;A61K31/16;A61K31/165;A61K31/221;A61K31/223;A61K31/341;A61K31/365;A61K31/381;A61K31/39;A61K31/421;A61K31/4409;A61K45/00;A61P31/00;A61P31/04;A61P31/06;A61P31/08;A61P43/00;C07D213/86;C07D263/10;C07D307/68;C07D409/12;(IPC1-7):A61K31/435;A61K31/335 |
主分类号 |
C07D333/32 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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