| 摘要 |
The present invention provides a process for producing virus-like particles, which can produce virus-like particles having the protein of a virus and a lipid bilayer membrane derived from an eukaryotic microorganism as an outer membrane in a short period of time in a form that is not contaminated with a vector virus, and which enables the virus-like particles to be used for various applications. The process of the present invention comprises: (a) incorporating a gene encoding a protein essential for viral particle budding or a fragment thereof into a vector which can be expressed in a eukaryotic microorganism; (b) transfecting the vector into the eukaryotic microorganism; (c) culturing a transformed eukaryotic microorganism; (d) removing a cell wall of the eukaryotic microorganism; and (e) further culturing, followed by collection of a culture supernatant.
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