发明名称 |
Targeted delivery of genes encoding secretory proteins |
摘要 |
Molecular complexes for targeting a gene encoding a secretory protein to a specific cell in vivo and obtaining secretion of the protein by the targeted cell are disclosed. An expressible gene encoding a desired secretory protein is complexed to a conjugate of a cell-specific binding agent and a gene-binding agent. The cell-specific binding agent is specific for a cellular surface structure which mediates internalization of ligands by endocytosis. An example is the asialoglycoprotein receptor of hepatocytes. The gene-binding agent is a compound such as a polycation which stably complexes the gene under extracellular conditions and releases the gene under intracellular conditions so that it can function within a cell. The molecular complex is stable and soluble in physiological fluids and can be used in gene therapy to selectively transfect cells in vivo to provide for production and secretion of a desired secretory protein.
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申请公布号 |
US2004009900(A1) |
申请公布日期 |
2004.01.15 |
申请号 |
US20030402267 |
申请日期 |
2003.03.28 |
申请人 |
WU GEORGE Y.;WILSON JAMES M.;WU CATHERINE H. |
发明人 |
WU GEORGE Y.;WILSON JAMES M.;WU CATHERINE H. |
分类号 |
A61K47/48;A61K48/00;C12N15/87;(IPC1-7):A61K48/00;C07K9/00 |
主分类号 |
A61K47/48 |
代理机构 |
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代理人 |
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地址 |
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