| 摘要 |
In one aspect, the disclosure concerns improved methods for the modification of genes in cells. Particular methods accomplish recombination, including targeted homologous recombination of a target gene. In another aspect, the disclosure relates to DNA modifying molecules. In one aspect, DNA modifying molecules comprise DNA targeting agents, including modified oligonucleotides, including triplex forming oligonucleotides, peptide nucleic acids and polyamides. In one embodiment, the DNA modifying molecules comprise a mutagen, and in another embodiment the DNA modifying molecules comprise a mutagen and a DNA targeting agent. The disclosure also describes methods for modifying a nucleotide sequence in the genome of a cell using the DNA targeting agents. The disclosure also relates to cells, tissue, and organisms that have been modified by DNA targeting agents.
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