NANOPARTICLE POLYANION CONJUGATES AND METHODS OF USE THEREOFIN DETECTING ANALYTES

摘要

This invention provides polyanionic polymer conjugates containing non- nucleotide polyanionic polymers that are useful in detecting target analytes such as proteins or small molecules. The invention also provides nanoparticl e bound to polyanionic polymer conjugates and methods of preparation and use thereof. The polyanionic polymer conjugates have the formula: L-O~PO2-O-Z-O~ n- PO2-O-X wherein n ranges from 1 to 200; L represents a moiety comprising a functional group for attaching the polyanion polymer to the nanoparticle surface; Z represents a bridging group, and X represents Q, X~ or -Q-X~, wherein Q represents a functional group for attaching a recognition probe to the polyanion polymer, and X~ represents a recognition probe.