摘要 |
Provided are a method and a system for allergy analysis by using RNA derived from leukocytes extracted from peripheral blood as such, correcting the observed value, and comparing the balance of helper T (Th) cells (Th1/Th2) with the number of Th1 cells/the number of Th2 cells in a nucleic acid sample or the Th1/Th2 data of a patient and of a normal volunteer. Also provided are a method and a system for allergy analysis using a highly reproducible and reliable array having a minimal number of DNA fragments (oligonucleotides) thereon, which is realized by identifying essential gene groups for the Th1/Th2 assay. According to the present invention, it is possible to analyze the existence of a specific cell in a sample containing several types of cells without isolating the cell of interest.
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