发明名称 |
Monoclonal antibody recognizing C-terminus of hBNP |
摘要 |
A hybridoma producing a monoclonal antibody recognizing the C-terminus of human brain natriuretic peptide (hBNP) was cultivated in a medium or the abdominal cavity of a mouse to recover the monoclonal antibody from the medium or ascites accumulated in the abdominal cavity. An immunoassay for hBNP was established using the monoclonal antibody. The immunoassay for hBNP of the invention is so sensitive that the minimum detection limit is 1 pg/ml and can therefore determine the hBNP level in blood plasma directly, without the extraction of hBNP from blood plasma. It is useful for diagnosing diseases such as hypertension and the like, and states of the heart, kidney, and the like by using the increase/decrease of the hBNP level as an index.
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申请公布号 |
US6677124(B2) |
申请公布日期 |
2004.01.13 |
申请号 |
US20010942709 |
申请日期 |
2001.08.31 |
申请人 |
SHIONOGI SEIYAKU KABUSHIKI KAISHA |
发明人 |
TSUJI TETSUO;INOUYE KEN;YAMAUCHI AKIRA;KONO MASAO;IGANO KEN'ICHI |
分类号 |
C12N5/10;C07K16/26;C12N15/02;C12N15/09;C12P21/08;C12R1/91;G01N33/53;G01N33/577;(IPC1-7):G01N33/53;G01N33/567;A61K39/395;A61K39/40;C07K16/00;C07K17/00 |
主分类号 |
C12N5/10 |
代理机构 |
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地址 |
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