| 摘要 |
PROBLEM TO BE SOLVED: To provide a detecting method and a detecting tip for biochemistry specimen which can detect a target gene without using a marker modification, and can simplify a detecting process with high reproducibility and does not require excessive skill of a measurer even when the marker modification is used. SOLUTION: Since a probe DNA forming a loop structure is hybridized only when a target DNA exists, hence the loop structure is eliminated and the probe DNA expands, the hybridized probe DNA is apparently different from a probe DNA keeping the loop structure in height from a substrate. The existence of the hybridization is detected as the height difference and a three-dimensional step. The hybridized probe DNA can be selectively modified by various markers, and the step between it and the probe DNA keeping the loop structure is enlarged by adding modified marker size to the height difference between the probe DNAs, and the existence of the hybridization is detected and identified. COPYRIGHT: (C)2004,JPO
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