发明名称 |
METHOD FOR THE SECRETION AND PRODUCTION OF PROTEIN |
摘要 |
<p>The object of the present invention is to provide a method of producing a heterologous protein by making a coryneform bacterium to produce and efficiently extracellularly secrete (secreto-production) an industrially useful heterologous protein. According to the present invention, a genetic construct is used where a gene sequence encoding an intended protein which is ligated to the downstream of a sequence encoding the signal peptide derived from a coryneform bacterium, the gene construct is introduced into a mutant coryneform bacterium which has a capacity of secreting the heterologous protein at least 2-fold higher than the wild type Corynebacterium glutamicum ATCC13869, the mutant coryneform bacterium is cultured and the extracellularly released heterologous protein is recovered.</p> |
申请公布号 |
EP1375664(A1) |
申请公布日期 |
2004.01.02 |
申请号 |
EP20020708684 |
申请日期 |
2002.03.27 |
申请人 |
AJINOMOTO CO., INC. |
发明人 |
KIKUCHI, YOSHIMI;DATE, MASAYO;UMEZAWA, YUKIKO;YOKOYAMA, KEIICHI;HEIMA, HARUO;MATSUI, HIROSHI |
分类号 |
C12N15/12;C07K5/107;C07K14/195;C07K14/34;C07K14/36;C12N1/21;C12N15/09;C12N15/21;C12N15/77;C12P21/02;C12R1/15;(IPC1-7):C12N15/21;C07K19/00;C07K7/08 |
主分类号 |
C12N15/12 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|