D-amino acid oxidase from Arthrobacter protophormiae

摘要

The invention relates to the microbial production of D-amino oxidase (D-AAO, EC 1.4.3.3). A nucleotide sequence encoding said enzyme has been isolated from Arthrobacter protophormiae and cloned. The coding sequence has been inserted into an expression construct which in turn has been inserted into a vector capable of transforming a microbial expression host. The transformed microbial hosts may be used to economically produce D-AAO. The D-AAO produced via the present invention may be used to oxidize D-amino acids.