| 摘要 |
1,166,917. Pullulanase. FARBWERKE HOECHST A.G. 6 Feb., 1967 [5 Feb., 1966], No. 5590/67. Heading C3H. A process for the manufacture of pullulanase by cultivation of Aerobacter aerogenes A.T.C.C. 15050 comprises cultivating the bacterium in a culture medium known per se and in the presence of a carbon source either (a) continuously or (b) discontinuously in the presence of a carbon source comprising glucose and maltose together, and isolating the enzyme from the cell mass. For (a) the carbon source may be maltose or pullulane or either of these plus glycerine. The enzyme is produced intracellularly and can be liberated by a surface-active agent, purified and isolated by known methods.
|
