Methods of identifying genes for the manipulation of triterpene saponins

摘要

The invention provides methods for the isolation of plant genes and their regulatory sequences involved in the biosynthesis of triterpene saponins. Also provided by the invention are genes involved in the biosynthesis of triterpenes, including squalene synthase, squalene epoxidase and beta-amyrin synthase from Medicago truncatula. The identification of triterpene biosynthesis genes allows genetic modification of the content and composition of triterpene saponins in plants for crop improvement and the development of drugs, nutriceuticals and functional foods.