| 摘要 |
The invention relates to a method for expressing a heterologous protein in E. coli cells while slowly shifting the temperature during the expression phase. According to the invention, the temperature is reduced 20 to 60 minutes, preferably 40 minutes, after induction in small steps of 0.2 to 0.8 DEG C, preferably 0.5 DEG C, at an interval of 10 to 30 minutes, preferably 20 minutes, from 35 DEG C to 26 DEG C. |
