GENETICALLY ENGINEERED KERATINOCYTES EXPRESSING AT LEAST ONE GROWTH FACTOR AND/OR ACTIVATED EGF RECEPTOR, SKIN EQUIVALENT COMPRISING SAID KERATINOCYTES AND TOXICITY ASSAY USING SAID KERATINOCYTES

摘要

Described is an immortalized mammalian skin keratinocyte line, preferably HaCaT, stably transfected with a vector capable of expressing a nucleic acid sequence encoding at least one growth factor and/or activated EGF receptor. Furthermore, a skin equivalent and an in vitro toxicity assay making use of this immortalized mammalian skin keratinocyte line is described.