METHOD FOR SEQUENCING NUCLEIC ACIDS

摘要

The present invention provides methods for performing sequencing by synthesis reactions. Some of the methods involve reducing the background noise level of sequencing by synthesis reactions by preventing nucleotide incorporation events that are not specific to the sequence being analyzed. Decreasing background results in longer read lengths in sequencing by synthesis methods. The present methods generally involve preventing extension of the 3' end of a target nucleic acid while performing the sequencing by synthesis reaction. Other methods involve the use of blocking agents to provide a defined endpoint in the sequencing reaction.