发明名称 |
STORING AND DETECTING NUCLEIC ACID ADMINISTERED TO A SOLID CARRIER |
摘要 |
The invention provides a method for detecting a nucleic acid of interest in at least one sample, comprising administering said sample to a solid carrier capable of at least in part absorbing said sample, drying said carrier, providing at least a representative part of said carrier to a nucleic acid isolation solution so that a representative amount of said nucleic acid is extracted from said carrier, and detecting said representative amount of said nucleic acid. With a method of the invention a sample, such as a body fluid sample, is stabilized in such a way that it can be shipped from the site of taking (for instance local hospital or lab in a less-developed country) and be sent to a service testing laboratory elsewhere in the world by normal logistics means. Preferably at least 100 µl , more preferably at least 250 µl of sample is administered to said carrier, in order to detect low titers of nucleic acid of interest. A kit of parts for detecting, identifying and/or quantifying a nucleic acid of interest in a sample, comprising a solid carrier capable of at least in part absorbing said sample, and a nucleic acid isolation solution, is also herewith provided. |
申请公布号 |
WO03080869(A3) |
申请公布日期 |
2003.12.31 |
申请号 |
WO2003NL00491 |
申请日期 |
2003.07.03 |
申请人 |
PRIMAGEN HOLDING B.V.;DE ROOIJ, ESTHER, REGINA;DE BAAR, MARINUS, PETRUS |
发明人 |
DE ROOIJ, ESTHER, REGINA;DE BAAR, MARINUS, PETRUS |
分类号 |
G01N33/53;C12M1/00;C12N15/09;C12Q1/68;C12Q1/70;G01N33/569 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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