NUCLEIC ACID ANALYSIS USING TYPE IS RESTRICTION ENDONUCLEASES

摘要

A method of analysing a nucleic acid sequence comprises digesting the sequence with a restriction enzyme which cleaves the nucleic acid to produce fragments with overhangs containing at least one partially random or at least one semi-random base. The fragment mixture may be analysed to determine the relative size of the fragments and the sequences of their ends. Alternatively the fragment mixture may be analysed to determine the identity of at least one of said random or semi-random bases and optionally to determine the relative size of the fragments to obtain sequence data that can be used to order the fragments relative to each other to generate partial or complete restriction maps of said nucleic acid sequence.