| 摘要 |
PURPOSE: Provided is a β-1,3 glucan producing microorganism which is a mutant of an Agrobacterium sp. strain and produces the microbial polysaccharide, β-1,3 glucan, in high yields, thereby increasing the production yield of β-1,3 glucan. CONSTITUTION: β-1,3 glucan producing microorganism, Agrobacterium sp. R259 KCTC 10197BP is provided, wherein Agrobacterium sp. R259 KCTC 10197BP shows blue in a medium containing aniline blue; and Agrobacterium sp. R259 KCTC 10197BP is produced by culturing Agrobacterium sp. ATCC 31750 in a YP broth medium at pH 7.0 and 30 deg. C for 15 to 17 hours; centrifuging the cultured medium and removing the supernatant; adding a citrate buffer solution and MNTG (N-methyl-N'-nitro-N-nitrosoguanidine) to the cell pellet and reacting them at 30 deg. C and 140 rpm for 1 hour; spreading MNTG treated bacterium on a YP agar medium containing aniline blue and culturing it at 30 deg. C for 48 hours; selecting colonies showing blue; culturing the colonies in a broth medium at pH 7.0 and 30 deg. C for 3 days; and selecting one strain showing the excellent polysaccharide producing activity.
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