| 摘要 |
PURPOSE: A composition and kit for identification of non-fermentative gram negative bacteria are provided, thereby identifying bacteria in the spleen, and diagnosing diseases caused by bacteria. CONSTITUTION: A method for identifying non-fermentative gram negative bacteria comprises the steps of: (a) preparing suspension of a microorganism to be identified; (b) reacting the suspension with compositions for testing sugar oxidization, beta-galactosidase activity, gelatinase activity, esculin hydrolysis, organic acid alkalization, urease activity, arginine dihydrolase activity, hydrogen sulfate production reaction, fermentation, nitric acid reduction, and indol production; and (c) analyzing the results of reactions. A composition for identification of non-fermentative gram negative bacteria comprises NH4H2PO4, MgSO4 7H2O, KCl, yeast extract, casamino acid, polypeptone, trace undiluted urine solution, CaCl2, undiluted vitamin solution, ferric ammonium salt citrate, BTB and sugars, wherein the trace undiluted urine solution comprises sodium molybdate, boric acid, cobalt chloride, zinc sulfate, manganese chloride, sodium selenite, nickel chloride and CuCl2; and the undiluted vitamin solution comprises vitamin B12, biotin, folic acid, riboflavin, nicotinic acid, lipoic acid, ρ-aminobenzoic acid, thiamine, calcium DL-pantothenate and pyridoxine.
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