Methods of characterizing molecular interaction sites on proteins

摘要

The present invention is directed to methods of characterizing molecular interaction sites on a biological target molecule. The methods involve contacting a polypeptide with a library of ligand candidates each of which is capable of tethering to the polypeptide at a site of interest, and analyzing the physicochemical properties of the ligand candidates tethering to the polypeptide. The methods herein are useful for discovery of and development of small molecule drugs.