发明名称 |
METHODS AND COMPOSITIONS FOR HIGH THROUGHPUT IDENTIFICATION OF PROTEIN/NUCLEIC ACID BINDING PAIRS |
摘要 |
Methods and compositions for high-throughput identification of protein/nucleic acid binding pairs are provided. In the subject methods, a nucleic acid probe array, e.g., a molecular beacon probe array, is contacted with a target nucleic acid population to produce a hybridized array. The resultant hybridized array is then contacted with a population of proteins to produce a protein bound array. Any resultant array surface bound target nucleic acid/protein complexes are then detected to identify protein/nucleic acid binding pairs. In certain embodiments, the protein and/or nucleic acid members of the identified protein/nucleic acid binding pairs are further characterized. Also provided are systems and kits for use in practicing the subject methods. The subject invention finds use in a variety of different applications.
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申请公布号 |
US2003235828(A1) |
申请公布日期 |
2003.12.25 |
申请号 |
US20020180437 |
申请日期 |
2002.06.25 |
申请人 |
GILLIBOLIAN ROBERT;LEPROUST ERIC M. |
发明人 |
GILLIBOLIAN ROBERT;LEPROUST ERIC M. |
分类号 |
C12Q1/68;C40B40/06;C40B60/14;(IPC1-7):C12Q1/68;C12M1/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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