METHOD FOR UTILIZING THE 5'END OF mRNA FOR CLONING AND ANALYSIS

摘要

A method is disclosed for obtaining the 5'ends of transcribed regions from a plurality of nucleic acid fragments obtained from biological materials or synthetic pools. DNA fragments encoding the 5'ends are enriched for their individual analysis or for the analysis of concatemers thereof. The sequence information derived from 5' ends can be used for characterization and cloning of the transcriptome.