BIOMOLECULE OPEN CHANNEL SOLID PHASE EXTRACTION SYSTEMS AND METHODS

摘要

An open capillary channel device for open tubular solid phase extraction of molecules capable of providing a tube enrichment factor of at least 1. The device comprises a channel (2, 12, 18, 42, 68, 98, 128, 200) having one end connected to a pump (44, 70,100, 218) for pumping liquid and gas, and the other end can be connected to an interface (26) for a protein chip sample applicator (32) or a mass spectrometer. The inner surface (4) of the channel, an extraction surface, can be bonded to an affinity binding agent such as a chelated metal, a protein, a sugar or nucleic acid. The method uses this device to bind analyte molecules from a sample solution to the affinity extraction surface and desorb analyte from the extraction surface with a desorbent liquid, with an extraction factor greater than 1.