| 摘要 |
<p>Disclosed is a method for the simultaneous detection of hybridization and vaccination reactions in a sample that is expected to contain target analytes which comprise at least one nucleic acid and at least one other ligand of a different type. Said method comprises the following steps: (i) a known volume of the diluted sample is introduced into a reaction buffer on a capturing surface which has previously been coated with the capturing partners of the target analytes, said capturing partners comprising at least one nucleic probe and at least one antiligand; (ii) a reaction is carried out at a temperature ranging between 15 °C and 60 °C; and (iii) the obtained hybridization and vaccination reactions are visualized. Also disclosed is the use of the inventive method for detecting infectious, metabolic, and viral diseases, for industrial diagnosis of the presence of bacteria, and for identifying and/or quantifying biological molecules. The invention further relates to the diagnostic use of said method and biological or diagnostic analysis kits that are used for carrying out the inventive method.</p> |
