发明名称 |
ELECTROPORATION DEVICE |
摘要 |
Electro-poration device for the permeabilization of cells (C) contained in a substrate (12) comprising a signal generator (3) for generating a stimulating signal (S(t)) applied by means of electrodes (6, 7) to the substrate (12) wherein an electric field (E(t)) permeabilizing the cells membranes is induced. The device calculates and monitors (15, 16, 23) the instantaneous value or the ratio GT between the current (ie) flowing through the substrate (12) and the voltage (Vp) of the stimulating signal (S(t)) applied to the substrate (12); in particular, the stimulating signal is applied in a modified manner according to the shape of an initial portion of the waveform of a curve CGT representing the value of ratio GT in successive instants after the application of the stimulating signal (S(t)). The system will achieve permeabilization of cells in substrate with no or minimum damage, allowing to introduce or to extract molecules in or from the electropermeabilized living cells.
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申请公布号 |
WO03104448(A1) |
申请公布日期 |
2003.12.18 |
申请号 |
WO2003EP05993 |
申请日期 |
2003.06.10 |
申请人 |
IGEA S.R.L.;MIKLAVCIC, DAMIJAN;MIR, LLUIS |
发明人 |
MIKLAVCIC, DAMIJAN;MIR, LLUIS |
分类号 |
C12M1/42;C12N13/00;C12N15/87;(IPC1-7):C12N13/00;C12M3/00 |
主分类号 |
C12M1/42 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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