摘要 |
Methods of performing array-based assays are provided. A feature of the subject methods is that a sample is initially contacted with a first substrate, i.e., a backing element, to produce a substrate supported sample. Next, the resultant substrate supported sample is contacted with an array of at least two different ligands, and the presence of any resultant surface bound binding complexes is detected. Also provided are backing elements, as well as kits and systems, for use in practicing the subject methods. The subject methods and compositions find use in any array based application, including genomic and proteomic applications.
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