Oligonucleotides labeled with stable isotopes and a method for detecting the same

摘要

Antisense oligonucleotide sequences which enable the measurement of the distribution and structure of antisense oligonucleotide drugs in the body, with lapse of time, and a method of detecting these sequences are provided. The antisense chains have a natural or non-natural nucleotide or peptide nucleic acid as a structure unit in which carbon atoms and nitrogen atoms are substituted by <13>C and <15>N, respectively, and the antisense chains can be detected by nuclear magnet resonance spectrometry (NMR) such as <15>N-<1>H or <13>C-<1>H heteronuclide multiple quantum coherence spectrometry.