发明名称 |
Genetically engineered L-sorbose reductase-deficient mutants |
摘要 |
The present invention provides a genetically engineered microorganism belonging to the genus Gluconobacter or Acetobacter, which has an engineered gene for the biological activity of reducing L-sorbose which is more than 90% non-functional in developing the said biological activity. The engineered microorganism is derived from a microorganism belonging to the genus Gluconobacter or Acetobacter, and has the biological activity for reducing L-sorbose that is less than 10% of the amount of the activity of the wild type organism. The present invention further provides a method for producing the genetically engineered microorganism by mutating the gene of a microorganism of the genus Gluconobacter or Acetobacter which encodes a protein having the L-sorbose reductase activity, determining the L-sorbose reductase activity of the resulting microorganism with the mutated gene and selecting the genetically engineered microorganism having L-sorbose reductase activity that is less than 10% of the amount of the activity of the wild type microorganism.
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申请公布号 |
US6664082(B1) |
申请公布日期 |
2003.12.16 |
申请号 |
US19990266320 |
申请日期 |
1999.03.11 |
申请人 |
ROCHE VITAMINS INC. |
发明人 |
HOSHINO TATSUO;KON TAKAHIDE;SHINJOH MASAKO;TAZOE MASAAKI |
分类号 |
C12N15/09;C12N1/21;C12N9/02;C12N15/10;C12P7/58;C12P19/02;C12R1/01;C12R1/02;(IPC1-7):C12P19/02;C12N9/04;C12N15/52;C12N15/87 |
主分类号 |
C12N15/09 |
代理机构 |
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地址 |
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