发明名称 |
RENILLA LUCIFERASE UND GREEN FLUORESCENT PROTEIN FUSIONSGENE |
摘要 |
A fusion gene is provided comprising the cDNA of Renilla luciferase and the cDNA of the "humanized" Aequorea green fluorescent protein. The fusion gene was used to produce a novel protein, the "Renilla-GFP fusion protein," which displayed both the luciferase activity of Renilla luciferase, and the green fluorescence of GFP. The Renilla-GFP fusion gene is useful as a double marker for monitoring gene expression quantitatively in UV light and by enzyme activity. |
申请公布号 |
AT254664(T) |
申请公布日期 |
2003.12.15 |
申请号 |
AT19970943558T |
申请日期 |
1997.09.24 |
申请人 |
LOMA LINDA UNIVERSITY |
发明人 |
SZALAY, ALDAR, A.;WANG, GEFU;WANG, YUBAO |
分类号 |
C12N15/09;C07K14/00;C07K14/435;C07K16/00;C07K16/40;C07K19/00;C12N9/02;C12N15/02;C12P21/04;C12P21/08;(IPC1-7):C12P21/04;C07H21/04;C12P21/06;C12N1/20 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|