| 摘要 |
<p>A method of counting microorganisms or cells in a sample by labeling the microorganisms or the cells with a fluorescent labeling, which comprises contacting the sample containing the microorganisms or the cells with an adhesive tape having an adhesive layer laminated on at least one face of a base material to thereby capture the sample; before fluorescent-labeling the microorganisms or the cells, obtaining a fluorescent image (a first image) of the sample; after fluorescent-labeling the microorganisms or the cells, obtaining another fluorescent image (a second image) of the sample; and then determining the difference (B-A) in the number of luminescent spots between the first and second images, or determining a differential image between the first and second images and determining the number of luminescent spots in this differential image, or determining the number of luminescent spots, among the luminescent spots in the second image, located outside non-sensitive regions attached to the individual luminescent spots in the first image. Thus, the effects of fluorescent contaminants are eliminated regardless of the form of a sample and changes in the positions of the microorganisms or the cells and contaminants in the sample are regulated so that the measurement accuracy can be improved and the measurement procedure can be simplified.</p> |
