| 摘要 |
The present invention relates to methods for monitoring interactions between target-molecules and at least one analyte comprising: (a) contacting a plurality of target-molecules, said target-molecules being immobilized on a porous substrate, with a sample, said sample comprising at least one analyte ; (b) incubating said target-molecules with said sample under conditions supporting target-molecule/analyte interaction; (c) monitoring said interaction as defined in step (b), said monitoring being in function of tim e of incubation as defined in step (b); (d) optionally determining at least on e interaction parameter; (e) varying said conditions as defined in step (b) by varying a reaction parameter; and; repeating, at least once, steps (c) to (e ). The present invention further relates to the uses of said methods as well as to the use of a porous substrate for the preparation of a microarray kit for carrying out said methods.
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