IMPROVED RECEPTOR DETECTION

摘要

Improved results are obtained using in an enzyme fragment complementation assay, as an enzyme donor a fragment of .beta.-galactosidase of from 36 to 5 0 amino acids joined to a ligand capable of binding to a receptor other than a n immunoglobulin or fragment thereof. Conveniently, for enzyme assays, enzyme binding site inhibitors are conjugated to the enzyme donor, whereby binding of the enzyme to the binding site inhibitor results in a reduction in the turnover rate of .beta.-galactosidase in the presence of EA and a substrate capable of producing a detectable signal. Analogously, ligands for membrane receptors may be conjugated to the ED for measurement of membrane receptors.